Journal: Advanced Science

Article Title: The Imbalance of p53–Park7 Signaling Axis Induces Iron Homeostasis Dysfunction in Doxorubicin‐Challenged Cardiomyocytes

doi: 10.1002/advs.202206007

Figure Lengend Snippet: Blocking myocardial ferroptosis significantly alleviates DoIC in vivo. a) Experiment design. A chronic DoIC mouse model was established in C57BL/6J mice, and Fer‐1, DFO, and DXZ were used for anti‐ferroptosis treatment during the DoIC model. All tests and analysis were implemented 2 weeks after the last DOX injection. b) Body weight of mice ( n = 10). c) Ratio of heart weight to tibia length (HW/TL; n = 6). d) Representative M‐mode images of transthoracic echocardiography, and quantification of left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS; n = 6). e) Representative micrographs of isolated AMCMs stained with cTnT (scale bars, 100 µm). f) Representative tracings of sarcomere length of isolated AMCMs using an Ionoptix HTS system, and calculation of basal myocyte length, maximum systolic velocity, maximum diastolic velocity, and percentage of shortening of AMCMs ( n = 6). g) Representative micrographs of intracellular iron level (FerroOrange staining), ROS (CellROS staining), lipid peroxide (Liperfluo staining), and mitochondrial membrane potential (TMRM staining) in AMCMs (scale bars, 20 µm), and the quantitative analysis of the fluorescence intensities were shown in (h–k) ( n = 6). l) Representative micrographs of heart tissues examined by transmission electron micrographs (scale bars, 500 nm). The data are expressed as mean ± SD and analyzed using one‐way ANOVA followed by Tukey's post hoc test, * p < 0.05, ** p < 0.01, and *** p < 0.001 versus Vehicle group; # p < 0.05, ## p < 0.01, and ### p < 0.001 versus DOX group.

Article Snippet: [ ] LVEF, LVFS, and GLS were analyzed using Vevo Analysis software (FUJIFILM Visual Sonics, Japan).

Techniques: Blocking Assay, In Vivo, Injection, Isolation, Staining, Membrane, Fluorescence, Transmission Assay

Journal: Advanced Science

Article Title: The Imbalance of p53–Park7 Signaling Axis Induces Iron Homeostasis Dysfunction in Doxorubicin‐Challenged Cardiomyocytes

doi: 10.1002/advs.202206007

Figure Lengend Snippet: Park7 restoration alleviates DOX induced myocardial ferroptosis in mice. a,i) Experiment design for chronic DoIC mouse model established in p53 CKO and Park7 OE mice. b,j) Quantification of LVEF and LVFS measured from M‐mode transthoracic echocardiography ( n = 6). c,k) Representative tracings of sarcomere length tracing of isolated AMCMs using an Ionoptix HTS system. d,l) The cellular and mitochondrial aconitase activity in AMCMs ( n = 6). e,m) Representative immunoblots of cellular and mitochondrial iron homeostasis–related proteins in AMCMs. f,n) Representative micrographs and quantification of intracellular iron level (FerroOrange staining) in AMCMs (scale bars, 20 µm, n = 6). g,o) Representative micrographs and quantification of mitochondrial iron level (Mito‐FerroGreen staining) in AMCMs (scale bars, 20 µm, n = 6). h,p) Representative micrographs of heart tissues examined by transmission electron micrographs (scale bars, 500 nm). The data are expressed as mean ± SD and analyzed using one‐way ANOVA followed by Tukey's post hoc test, ** p < 0.01 and *** p < 0.001 versus Vehicle group; $ p < 0.05, $$ p < 0.01, and $$$ p < 0.001 versus p53 f/f /Cre − +DOX group; @ p < 0.05 and @@@ p < 0.001 versus AAV9‐Vector+DOX group.

Article Snippet: [ ] LVEF, LVFS, and GLS were analyzed using Vevo Analysis software (FUJIFILM Visual Sonics, Japan).

Techniques: Isolation, Activity Assay, Western Blot, Staining, Transmission Assay, Plasmid Preparation